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KMID : 0545120120220070923
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 7 p.923 ~ p.929
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Optimization of Culture Conditions and Bench-Scale Production of L-Asparaginase by Submerged Fermentation of Aspergillus terreus MTCC 1782
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Baskar Gurunathan
Renganathan Sahadevan
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Abstract
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Abstract Optimization of culture conditions for L-asparaginase production by submerged fermentation of Aspergillus terreus MTCC 1782 was studied using a 3-level central composite design of response surface methodology and artificial neural network linked genetic algorithm. The artificial neural network linked genetic algorithm was found to be more efficient than response surface methodology. The experimental L-asparaginase activity of 43.29 IU/ml was obtained at the optimum culture conditions of temperature 35oC, initial pH 6.3, inoculum size 1% (v/v), agitation rate 140 rpm, and incubation time 58.5 h of the artificial neural network linked genetic algorithm, which was close to the predicted activity of 44.38 IU/ml. Characteristics of L-asparaginase production by A. terreus MTCC 1782 were studied in a 3 L bench-scale bioreactor.
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KEYWORD
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Aspergillus terreus, Asparaginase, Response surface methodology, Artificial neural network, Bench scale bioreactor
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